| A. E. Gardiner Building / Seton Centre | Carberry | 116 Main Street |
| Air Force Heritage Museum and Air Park | Winnipeg | Air Force Way |
| Alonsa School No. 1920 / Alex Robertson Museum / Alonsa War Memorial | Alonsa | Alonsa |
| Altona and District Heritage Research Centre | Altona | 125 Centre Avenue East |
| Angusville Municipal Building / Angusville Museum | Riding Mountain West | Angusville |
| Anola and District Museum | Springfield | 725 Weiser Crescent, Anola |
| Arborg and District Multicultural Heritage Village | Arborg | |
| Archibald Historical Museum | Pembina | |
| Ashern Pioneer Museum | West Interlake | Ashern |
| Assiniboia Municipal Hall / St. James-Assiniboia Historical Museum | Winnipeg | 3180 Portage Avenue |
| Badger Creek Museum / Cartwright Heritage Park | Cartwright-Roblin | Cartwright |
| Bank of Hamilton Building / Roland 4-H Museum | Roland | 72 Third Street, Roland |
| Bank of Toronto Building / Transcona Municipal Hall / Transcona Historical Museum / Transcona Museum | Winnipeg | 141 Regent Avenue W |
| Beautiful Plains Archives | Neepawa | 282 Hamilton Street |
| Beckoning Hills Museum | Boissevain-Morton | 425 Mill Road, Boissevain |
| Belmont and District Historical Museum | Prairie Lakes | Belmont |
| Boissevain-Morton Library & Archives | Boissevain-Morton | 409 South Railway Street, Boissevain |
| Brandon Armoury / 26th Field Regiment Museum | Brandon | 1116 Victoria Avenue |
| Brandon Fire and Emergency Services Museum | Brandon | 120 Nineteenth Street North |
| Brokenhead-Beausejour Pioneer Village Museum | Beausejour | |
| Buffalo Point Museum and Government Centre | Buffalo Point First Nation | |
| Burrough of the Gleann Museum | Glenboro-South Cypress | Broadway Street, Glenboro |
| Cameron Brothers Radio Store / Neal’s Grocery No. 11 / Ogniwo Polish Museum Society | Winnipeg | 1417 Main Street |
| Canadian Bank of Commerce Building / Elgin and District Museum | Grassland | 127 Main Street, Elgin |
| Canadian Museum for Human Rights | Winnipeg | 85 Israel Asper Way |
| Canadian National Railway Station / Beautiful Plains Museum | Neepawa | Fifth Avenue |
| Canadian National Railway Station / Cranberry Portage Heritage Museum | Kelsey | Cranberry Portage |
| Canadian National Railway Station / Dauphin Railway Museum | Dauphin (City) | 101 First Street NW |
| Canadian National Railway Station / Flin Flon Station Museum | Flin Flon | |
| Canadian National Railway Station / Lundar Museum | Coldwell | Railway Avenue, Lundar |
| Canadian National Railway Station / McCreary Museum | McCreary | Railway Street, McCreary |
| Canadian National Railway Station / Miami Railway Station Museum | Thompson (RM) | Letain Street, Miami |
| Canadian National Railway Station / Rolling Memories Museum | Fisher | Fisher Branch |
| Canadian National Railway Station / Winnipegosis Museum | Mossey River | First Street, Winnipegosis |
| Canadian Pacific Railway Station / Riverton Transportation and Heritage Centre | Bifrost-Riverton | Riverton |
| Canadian Pacific Railway Station / Strathclair Museum | Yellowhead | Strathclair |
| Carberry Plains Museum | Carberry | 520 Fourth Avenue |
| Carnegie Library / City of Winnipeg Archives | Winnipeg | 380 William Avenue |
| Casa Loma Building / Pinewood Museum | Riding Mountain National Park | Wasagaming |
| Centreville School No. 606 | Victoria | |
| Chapman Museum | Riverdale | |
| Charlebois Heritage Museum | The Pas | 108 First Street W |
| Charleswood Municipal Hall / Charleswood Community Museum | Winnipeg | 5006 Roblin Boulevard |
| Chatfield Park of Souvenirs Museum | Armstrong | Chatfield |
| Clack Family Museum | Oakview | |
| Clearwater Agricultural Museum | Louise | Clearwater |
| Convent of the Sisters of the Holy Names of Jesus and Mary / St-Pierre-Jolys Museum | St-Pierre-Jolys | 432 Joubert Street |
| Cooks Creek Heritage Museum | Springfield | Cooks Creek |
| Cowan School No. 1132 | Mountain | |
| Crystal City Printing Museum | Louise | 218 Broadway Street South, Crystal City |
| Cypress River Consolidated School No. 2357 / Cypress River Museum | Victoria | Cypress River |
| Dairy Museum of Manitoba | Grey | St. Claude |
| Daly House / The Maples Orphanage / Daly House Museum | Brandon | 122 Eighteenth Street |
| Darlingford Consolidated School No. 1228 / Darlingford School Heritage Museum / Darlingford Pembina Heritage Museum | Pembina | Darlingford |
| Dominion Post Office Building / Carberry-North Cypress Library / Carberry Plains Archives | Carberry | 115 Main Street |
| Dufferin Historical Society Museum | Carman | 44 Kings Park Road |
| Dunnottar Station Museum | Dunnottar | Ponemah |
| English Museum | Riverdale | |
| Episcopal Methodist Church / All Saints Anglican Church / Franklin Museum | Emerson-Franklin | Dominion City |
| Erickson Cabin / Lac du Bonnet and District Museum | Lac du Bonnet (RM) | |
| Eriksdale Creamery Museum | West Interlake | Eriksdale |
| Eskimo Museum / Itsanitaq Museum | Churchill | La Verendrye Avenue |
| Ethelbert and District Museum | Ethelbert | Ethelbert |
| Fire Hall No. 3 / Fire Fighters Museum of Winnipeg | Winnipeg | 56 Maple Street |
| Fort Dauphin Museum | Dauphin (City) | 140 Jackson Street |
| Fort la Reine Museum | Portage la Prairie (City) | Saskatchewan Avenue East |
| Foxwarren Museum | Prairie View | Foxwarren |
| Frame House / Virden Pioneer Home Museum | Virden | 390 King Street West |
| Gabrielle Roy House Museum | Winnipeg | 375 Rue Deschambault |
| Gardenton Museum | Stuartburn | Gardenton |
| Gilbert Plains Museum | Gilbert Plains | Gilbert Plains |
| Gladstone and District Museum | WestLake-Gladstone | 49 Sixth Street, Gladstone |
| Gordon Orr Memorial Museum | Russell-Binscarth | 152 Second Avenue, Binscarth |
| Grey Nuns Convent / Le Musée de Saint-Boniface Museum | Winnipeg | Tache Avenue |
| Grosse Isle Museum | Rockwood | Grosse Isle |
| Hamiota Pioneer Club Museum | Hamiota | Seventh Street South, Hamiota |
| Headingley Heritage Centre / Jim's Vintage Garages | Headingley | |
| Health Sciences Centre Archives and Museum | Winnipeg | 820 Sherbrook Street |
| Heamen's Antique Autorama | Dufferin | |
| Heritage North Museum | Thompson (City) | 162 Princeton Drive |
| Hill Building / Hart-Cam Museum | Grassland | 310 Poplar Street, Hartney |
| Holy Trinity Roman Catholic Church / Tolstoi Heritage Centre | Emerson-Franklin | Tolstoi |
| Holy Trinity Ukrainian Orthodox Cathedral / Ukrainian Museum of Canada - Manitoba Branch | Winnipeg | 1175 Main Street |
| Hryhoruk Museum | Mossey River | 225 Edward Street, Winnipegosis |
| Hudson Bay House / Winnipeg Skating Club / Winnipeg Winter Club / HMCS Chippawa / Naval Museum of Manitoba | Winnipeg | 51 Smith Street |
| Inglis Grain Elevators National Historic Site | Riding Mountain West | Inglis |
| Isabella Museum | Prairie View | Isabella |
| J. A. V. David Museum | Killarney-Turtle Mountain | 414 Williams Avenue, Killarney |
| Jewish Heritage Centre of Western Canada | Winnipeg | 123 Doncaster Street |
| J. R. Amos Blacksmith Shop | Brenda-Waskada | 101 Second Street, Waskada |
| Keystone Pioneers Museum | Roblin | |
| Lawlor House / Woodworth Millennium Museum | Wallace-Woodworth | Kenton |
| Leaf Rapids National Exhibition Centre | Leaf Rapids | |
| Living Prairie Museum | Winnipeg | Ness Avenue |
| Lockport Provincial Heritage Park / Kenosewun Centre and Museum | St. Clements | Lockport |
| Log Cabin Tourist Centre / Nellie McClung Heritage Site | Pembina | Main Street, Manitou |
| Lynn Lake Mining Town Museum | Lynn Lake | |
| Macdonald Antique Road Equipment | Macdonald | Sanford |
| Macdonald House / Dalnavert Museum | Winnipeg | 61 Carlton Street |
| Manitoba Agricultural Museum | North Norfolk | Austin |
| Manitoba Amateur Radio Museum | North Norfolk | Austin |
| Manitoba Antique Auto Museum | Wallace-Woodworth | Elkhorn |
| Manitoba Baseball Hall of Fame and Museum | Morden | 111B Gilmour Street |
| Manitoba Crafts Museum and Library | Winnipeg | 329 Cumberland Avenue |
| Manitoba Government Telephones Building / Brandon General Museum and Archives | Brandon | 19 Ninth Street |
| Manitoba Historical Society War Memorial | Winnipeg | |
| Manitoba Museum of Man and Nature / Manitoba Museum | Winnipeg | 190 Rupert Avenue |
| Manitoba Pool Grain Elevator / Plum Coulee and District Museum | Rhineland | Plum Coulee |
| Manitoba World War I Museum | Louise | |
| Marconi School No. 2085 | Rossburn | |
| Marine Museum of Manitoba | Selkirk | 490 Eveline Street |
| McGregor Armoury / Lieutenant-Colonel Harcus Strachan VC MC Armoury / Fort Garry Horse Museum and Archives | Winnipeg | 551 Machray Avenue |
| Medd House Museum | Mossey River | 324 Second Street, Winnipegosis |
| Medicine Rock Heritage Park | St. Francois Xavier | St. Francois Xavier |
| Melita School No. 440 / Antler River Museum | Melita | 41 Ash Street |
| Mennonite Heritage Centre Archives / Mennonite Educators Monument | Winnipeg | 600 Shaftesbury Boulevard |
| Mennonite Village Museum / Mennonite Heritage Village | Steinbach | Highway 12 |
| Messner Store Museum | Killarney-Turtle Mountain | Holmfield |
| Midwinter School No. 1891 / Midwinter School Heritage Museum | Reynolds | East Braintree |
| Miniota Municipal Museum | Prairie View | |
| Minnedosa and District Museum | Minnedosa | |
| Minnedosa Regional Archives | Minnedosa | 45 First Avenue SW |
| Minto Armoury | Winnipeg | 969 St. Matthews Avenue |
| Moosehorn Heritage Museum | Grahamdale | Moosehorn |
| Morden and District Museum / Canadian Fossil Discovery Centre | Morden | 111B Gilmour Street |
| Morris and District Centennial Museum | Morris (Town) | 350 Main Street South |
| Musee des Pionniers et des Chanoinesses | Lorne | Notre Dame de Lourdes |
| New Iceland Heritage Museum | Gimli | Gimli |
| No. 12 Service Flying Training School / Commonwealth Air Training Plan Museum | Brandon | 300 Commonwealth Way |
| Northern Grain Elevator / National Grain Elevator / Cargill Grain Elevator | Riding Mountain West | Inglis |
| Northern Pacific and Manitoba Railway Repair Shop / Manitoba Children's Museum | Winnipeg | 45 Forks Market Road |
| North Norfolk - MacGregor Archives | North Norfolk | 35 Hampton Street East, MacGregor |
| Nutimik Lake Museum | Whiteshell Provincial Park | |
| Oak Lake and District Museum | Sifton | 396 Aspen Street, Oak Lake |
| Paterson Grain Elevator | Riding Mountain West | Inglis |
| Pembina Threshermen's Museum | Stanley | |
| Pilot Mound and District Museum | Louise | 213 Lorne Avenue, Pilot Mound |
| Poole Building / Argyle Museum | Argyle | Baldur |
| Portage la Prairie Indian Residential School / National Indigenous Residencial School Museum of Canada | Portage la Prairie (City) | Crescent Road West |
| Prairie Dog Central Railway | Winnipeg | 1 Prairie Dog Trail |
| Prairie Mountain Regional Museum | Yellowhead | |
| Province Grain Elevator / Reliance Grain Elevator A / Manitoba Pool Grain Elevator A / United Grain Growers Grain Elevator 2 | Riding Mountain West | Inglis |
| Qually Brothers Store | Cartier | Dacotah |
| Quarry Park Heritage Arts Centre | Stonewall | 166 Main Street |
| Rapid City School No. 124 | Oakview | Rapid City |
| Reliance Grain Elevator B / Manitoba Pool Grain Elevator B / United Grain Growers Grain Elevator 3 | Riding Mountain West | Inglis |
| Reston and District Museum | Pipestone | Ninth Street, Reston |
| Riel House | Winnipeg | 330 River Road |
| Roland Antique Tractor Club and Agricultural Museum | Roland | Roland |
| Rossburn Museum | Rossburn | Rossburn |
| Ross House Museum | Winnipeg | 140 Meade Street N |
| Sandilands Forest Discovery Centre | Reynolds | Sandilands Provincial Forest |
| Satterthwaite Heritage Log Cabin | McCreary | |
| Selo Ukraina Museum | Dauphin (RM) | |
| Settlers, Rails and Trails Museum | Rockwood | Argyle |
| Seven Oaks House Museum | Winnipeg | 115 Rupertsland Boulevard E |
| Shoal Lake Police and Pioneer Museum | Yellowhead | Lakeview Park, Shoal Lake |
| Simpson House / Margaret Laurence House | Neepawa | 312 First Avenue |
| Site Historique Enfant-Jésus Heritage Site / Thibeaultville Cemetery / Musée Dawson Trail Museum | Ste. Anne (RM) | Richer |
| S. J. McKee Archives / Brandon University Archives | Brandon | 270 Eighteenth Street |
| Smart Bag Company Building / Manitoba Sports Hall of Fame and Museum | Winnipeg | 145 Pacific Avenue |
| Snow Lake Mining Museum | Snow Lake | Poplar Avenue |
| Souris Fire Department Museum | Souris-Glenwood | Sowden Street South, Souris |
| Souris Hillcrest Community Museum | Souris-Glenwood | 26 Crescent Avenue E, Souris |
| Split Lake Heritage Centre | Tataskweyak Cree Nation | Split Lake |
| Sprague and District Historical Museum | Piney | Sprague |
| Springfield Archives | Springfield | Dugald |
| St. Andrew's Presbyterian Church / St. Andrew's United Church / Arden Museum | Glenella-Lansdowne | Arden |
| Stationary Engine Museum | Killarney-Turtle Mountain | |
| St. Boniface Fire Hall No. 1 | Winnipeg | 212 Dumoulin Street |
| St. Claude and Area Archives | Grey | 50 First Street East, St. Claude |
| Ste. Genevieve Roman Catholic Church and Cemetery / Monseigneur Tache Historic Site / Old Rectory Museum | Tache | 98 rue Saltel, Ste-Genevieve |
| St. Elijah Romanian Orthodox Church / Pioneer Museum and Paulencu House | Riding Mountain West | Lennard |
| St. Georges Museum / Winnipeg River Heritage Museum | Alexander | St. Georges |
| St. James Anglican Church / Miami Museum | Thompson (RM) | 99 Third Street, Miami |
| St. John's Anglican Church / Eriksdale Museum | West Interlake | Eriksdale |
| St. John's Anglican Church / St. John's Heritage Church and Arts Centre | Lac du Bonnet (Town) | 86 McArthur Avenue |
| St. Joseph Museum | Montcalm | St. Joseph |
| St. Leon Interpretive Centre | Lorne | St. Leon |
| St. Luke's Anglican Church / The Plum Museum and Tea Room | Souris-Glenwood | 142 First Street S, Souris |
| St. Malo Museum | De Salaberry | St. Malo |
| St. Margaret's Anglican Church | Mountain | Mafeking |
| St. Norbert Provincial Heritage Park | Winnipeg | Turnbull Drive |
| St. Vital Museum / St. Vital Fire Hall | Winnipeg | 600 St. Mary's Road |
| St. Vladimir Ukrainian Greek Orthodox Church / Russell Missouri Lutheran Church / Russell Heritage Church Museum | Russell-Binscarth | 203 Assiniboine Street, Russell |
| St. Volodymyr Ukrainian Catholic Archeparchial Museum and Archbishop's Chancery | Winnipeg | 233 Scotia Street |
| Swan Valley Historical Museum | Swan River | |
| Teulon and District Museum | Teulon | Green Acres Park |
| The Pas Community Building and Court House / Sam Waller Museum | The Pas | 306 Fischer Avenue |
| The Royal Aviation Museum of Western Canada | Winnipeg | 2088 Wellington Avenue |
| The Royal Canadian Artillery Museum | North Cypress-Langford | Shilo |
| The Royal Winnipeg Rifles Museum and Archives | Winnipeg | 969 St. Matthews Avenue |
| Third Crossing Agricultural Museum | WestLake-Gladstone | Gladstone |
| Treherne Museum | Norfolk Treherne | 183 Vanzile Street, Treherne |
| Treherne Second Chance Auto Museum | Norfolk Treherne | Treherne |
| Trembowla Cross of Freedom Museum | Dauphin (RM) | |
| Turtle River Relics | Ste. Rose | |
| Twilley Museum | Minitonas-Bowsman | |
| Ukrainian Cultural and Educational Centre / Oseredok | Winnipeg | 184 Alexander Avenue East |
| Ukrainian Cultural Heritage Museum | Harrison Park | Sandy Lake |
| Ukrainian Labour Temple / Ivan Franko Museum | Winnipeg | 591 Pritchard Avenue |
| Union Bank Building / Birdtail Country Museum | Prairie View | 738 Main Street, Birtle |
| Union Bank Building / Hamiota and District Archive | Hamiota | 39 Maple Avenue East, Hamiota |
| Union Bank Building / Royal Bank Building / Somerset Lorne Citizens Museum | Lorne | 302 Third Street, Somerset |
| Union Bank Building / Waskada Museum | Brenda-Waskada | 53 Railway Avenue, Waskada |
| United Grain Growers Grain Elevator | Riding Mountain West | Inglis |
| University of Manitoba Archives and Special Collections | Winnipeg | 25 Chancellors Circle |
| Wabowden Historical Museum | Northern Manitoba | Wabowden |
| Wasyl Negrych Pioneer Homestead | Gilbert Plains | |
| Watson Crossley Community Museum | Grandview | Grandview |
| Wawanesa Mutual Insurance Building / Sipiweske Museum | Oakland-Wawanesa | 102 Fourth Street, Wawanesa |
| West Hawk Museum | Whiteshell Provincial Park | West Hawk Lake |
| Whitemouth Municipal Museum | Whitemouth | Whitemouth |
| Whiteshell Trappers Museum | Whiteshell Provincial Park | Rennie |
| Winnipeg Art Gallery | Winnipeg | 300 Memorial Boulevard |
| Winnipeg Auditorium / Archives of Manitoba / Manitoba Legislative Library | Winnipeg | 200 Vaughan Street |
| Winnipeg Electric Company Harrow Substation / Manitoba Electrical Museum | Winnipeg | 680 Harrow Street |
| Winnipeg Police Museum | Winnipeg | 245 Smith Street |
| Winnipeg Railway Museum | Winnipeg | 123 Main Street |
| Woodlands Pioneer Museum | Woodlands | Woodlands |